IFI-K · Glutathione Enhancement

Specimen SS-F-KOK-213 // KOKUMI · IFI-K

Glutathione Enhancement II-PR-A

Allium sativum

Garlic

Tier IIGlutathione Enhancement

Target Trait

2.25×

fold-change

GSH biosynthesis fold-change via GCL/GS overexpression

Technical Specifications

SKU

SS-F-KOK-213

Genomic Edits

5

GC Content

44.6%

Construct Length

3,183 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

225

Pathway Edits

  • GCL (γ-glutamylcysteine synthetase) catalytic subunit overexpression cassette insertion
  • GS (glutathione synthetase) promoter swap to constitutive UBI1 for persistent expression
  • GSR (glutathione reductase) copy-number amplification for sustained NADPH-dependent recycling
  • gamma-ECS intermediate channel optimization to prevent rate-limiting precursor accumulation
  • Gly-I (glyoxalase I) competitive cytosolic drain silencing via targeted RNAi
  • Vacuolar GSH export transporter upregulation for cytosolic pool expansion
  • GGT1 retrograde hydrolysis attenuation to prevent GSH catabolism by transpeptidase

Key Interventions

GCL/GS co-overexpression for direct GSH pool amplification targeting CaSR γ-glutamyl substrateGSR recycling loop enhancement for sustained reduced glutathione availability at CaSR binding sitesgamma-ECS intermediate accumulation to load CaSR γ-glutamyl peptide kokumi substrate poolProtoplast PEG-fusion delivery optimized for Allium sativum genome accessibilityTier-II construct validated with 5 precision edits for 2.25× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
225
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Broth and stock kokumi foundation for mild mouthfulness enhancement in pilot formulations

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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