IFI-K · Glutathione Enhancement

Specimen SS-F-KOK-208 // KOKUMI · IFI-K

Glutathione Enhancement I-IV-B

Allium sativum

Garlic

Tier IGlutathione Enhancement

Target Trait

1.62×

fold-change

GSH biosynthesis fold-change via GCL/GS overexpression

Technical Specifications

SKU

SS-F-KOK-208

Genomic Edits

5

GC Content

46%

Construct Length

3,267 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

162

Pathway Edits

  • GCL (γ-glutamylcysteine synthetase) catalytic subunit overexpression cassette insertion
  • GS (glutathione synthetase) promoter swap to constitutive UBI1 for persistent expression
  • GSR (glutathione reductase) copy-number amplification for sustained NADPH-dependent recycling
  • gamma-ECS intermediate channel optimization to prevent rate-limiting precursor accumulation
  • Gly-I (glyoxalase I) competitive cytosolic drain silencing via targeted RNAi
  • Vacuolar GSH export transporter upregulation for cytosolic pool expansion
  • GGT1 retrograde hydrolysis attenuation to prevent GSH catabolism by transpeptidase

Key Interventions

GCL/GS co-overexpression for direct GSH pool amplification targeting CaSR γ-glutamyl substrateGSR recycling loop enhancement for sustained reduced glutathione availability at CaSR binding sitesgamma-ECS intermediate accumulation to load CaSR γ-glutamyl peptide kokumi substrate poolCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-I construct validated with 5 precision edits for 1.62× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
162
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Entry-level GSH kokumi ingredient for savory snack prototyping and R&D library screening

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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