IFI-F · Full-Spectrum Minimal Footprint Series

Specimen SS-F-IFC-080 // FULL SPECTRUM · IFI-F

Full-Spectrum Minimal Footprint Series V-IV

Stevia rebaudiana

Stevia

Tier VFull-Spectrum Minimal Footprint Series

Target Trait

4.5×

fold-change

IFI-F composite with minimal genomic edits

Technical Specifications

SKU

SS-F-IFC-080

Genomic Edits

8

GC Content

44.2%

Construct Length

5,485 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

All 8 IFI receptors (H, P, C, U, A, S, B, K)

IFI Score

450

Pathway Edits

  • Multi-axis pleiotropic master regulatory gene driving all 8 IFI pathway transcription networks
  • Bifunctional terpene synthase serving both TRPV1 (heat) and TRPM8 (cooling) receptor targets
  • Dual amino acid pathway gene for simultaneous umami (glutamate) and kokumi (glutathione) accumulation
  • Combinatorial polyphenol pathway gene for simultaneous astringency and bitter-attenuation outputs
  • Multi-functional glycosyltransferase for sweetness + astringency profile optimization

Key Interventions

Minimal-edit multi-axis strategy: pleiotropic gene integrations serve multiple IFI receptor targets per transgeneBifunctional enzyme engineering for simultaneous heat/cooling terpene receptor axis coverage from one geneCombinatorial polyphenol pathway gene for simultaneous astringency and bitter-attenuation without additional integrationsCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
450

Use Case

Tier V Full-Spectrum Minimal Footprint construct for regulatory-sensitive applications (novel food filings, GMO labeling jurisdictions, precision fermentation deployments) where minimizing genomic edit count without sacrificing IFI-F composite output is the primary engineering objective.

License

$10499 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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