Full-Spectrum Minimal Footprint Series I-PR

Stevia rebaudiana

Stevia

Tier IFull-Spectrum Minimal Footprint Series

Target Trait

1.8×

fold-change

IFI-F composite with minimal genomic edits

Technical Specifications

SKU

SS-F-IFC-063

Genomic Edits

GC Content

48.9%

Construct Length

4,210 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

All 8 IFI receptors (H, P, C, U, A, S, B, K)

IFI Score

180

Pathway Edits

Multi-axis pleiotropic master regulatory gene driving all 8 IFI pathway transcription networks
Bifunctional terpene synthase serving both TRPV1 (heat) and TRPM8 (cooling) receptor targets
Dual amino acid pathway gene for simultaneous umami (glutamate) and kokumi (glutathione) accumulation
Combinatorial polyphenol pathway gene for simultaneous astringency and bitter-attenuation outputs

Key Interventions

Minimal-edit multi-axis strategy: pleiotropic gene integrations serve multiple IFI receptor targets per transgeneBifunctional enzyme engineering for simultaneous heat/cooling terpene receptor axis coverage from one geneCombinatorial polyphenol pathway gene for simultaneous astringency and bitter-attenuation without additional integrationsPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

180

Use Case

Tier I Full-Spectrum Minimal Footprint construct for regulatory-sensitive applications (novel food filings, GMO labeling jurisdictions, precision fermentation deployments) where minimizing genomic edit count without sacrificing IFI-F composite output is the primary engineering objective.

License

$799 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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