IFI-C · TRPM8 Antagonist-Platform

Specimen SS-F-CLB-863 // COOL BURN · IFI-C

TRPM8 Antagonist-Platform III-IV-A

Capsicum chinense

Pepper

Tier IIITRPM8 Antagonist-Platform

Target Trait

1.5 ratio

TRPM8/TRPV1 ratio

TRPM8/TRPV1 activation ratio

Technical Specifications

SKU

SS-F-CLB-863

Genomic Edits

6

GC Content

43.8%

Construct Length

3,644 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

150

Pathway Edits

  • AT-KO (TRPV1 antagonist peptide knockout) loss-of-function to relieve TRPM8-opposing TRPV1 tone
  • CREB3 (cyclic AMP response element binding protein 3) cooling transcription factor overexpression
  • CS2-RNAi (calcium-sensing receptor 2 RNAi) knockdown to potentiate TRPM8 signaling cascade
  • ANO1 (anoctamin 1 chloride channel) suppression to reduce thermal-nociceptive competing signal
  • TRPM8 promoter potentiation cassette for enhanced cooling channel expression
  • TRPV1 downregulation cassette for selective TRPM8/TRPV1 ratio shifting

Key Interventions

TRPM8/TRPV1 ratio shifting via TRPV1 downregulation and TRPM8 potentiation in Capsicum chinenseSustained cooling without thermosensory burn via ANO1/CS2-RNAi competitive channel suppressionCREB3 cooling transcription factor activation for persistent TRPM8-dominant sensory programming

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
150
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium TRPM8-dominant Capsicum ingredient supply, pharmaceutical pain-relief TRPM8 agonist ingredient development, functional food sustained-cooling ingredient engineering, and novel burn-free hot pepper platform creation.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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