IFI-C · D-Limonene Cooling

Specimen SS-F-CLB-664 // COOL BURN · IFI-C

D-Limonene Cooling III-IV-B

Citrus sinensis

Orange

Tier IIID-Limonene Cooling

Target Trait

2.01×

fold-change

D-limonene accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-664

Genomic Edits

6

GC Content

48.9%

Construct Length

3,661 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

201

Pathway Edits

  • LS (limonene synthase) codon-optimized overexpression in Citrus sinensis flavedo
  • GPPS (geranyl pyrophosphate synthase) upregulation for GPP precursor flux
  • DXS (1-deoxy-D-xylulose 5-phosphate synthase) MEP pathway rate-limiting amplification
  • HDR (4-hydroxy-3-methylbut-2-enyl diphosphate reductase) MEP pathway co-upregulation

Key Interventions

D-limonene TRPM8-weak/TRPA1-dual overproduction via LS/GPPS co-upregulation in Citrus sinensisMEP pathway amplification via DXS/HDR for enhanced IPP/DMAPP precursor availabilityTRPM8/TRPA1 dual-modality cooling activation with characteristic citrus aroma co-production

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
201
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium citrus oil D-limonene production, pharmaceutical-grade limonene ingredient supply, functional food citrus-cooling ingredient development, and aromatic TRPM8 platform creation.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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