IFI-C · D-Limonene Cooling

Specimen SS-F-CLB-656 // COOL BURN · IFI-C

D-Limonene Cooling II-IV-B

Citrus sinensis

Orange

Tier IID-Limonene Cooling

Target Trait

1.59×

fold-change

D-limonene accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-656

Genomic Edits

5

GC Content

47.3%

Construct Length

3,114 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

159

Pathway Edits

  • LS (limonene synthase) codon-optimized overexpression in Citrus sinensis flavedo
  • GPPS (geranyl pyrophosphate synthase) upregulation for GPP precursor flux
  • DXS (1-deoxy-D-xylulose 5-phosphate synthase) MEP pathway rate-limiting amplification
  • HDR (4-hydroxy-3-methylbut-2-enyl diphosphate reductase) MEP pathway co-upregulation

Key Interventions

D-limonene TRPM8-weak/TRPA1-dual overproduction via LS/GPPS co-upregulation in Citrus sinensisMEP pathway amplification via DXS/HDR for enhanced IPP/DMAPP precursor availabilityTRPM8/TRPA1 dual-modality cooling activation with characteristic citrus aroma co-production

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
159
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Citrus flavor enhancement, D-limonene ingredient sourcing, citrus-cooling beverage formulation, and functional food applications requiring natural TRPM8/TRPA1 dual-activation with citrus character.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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