IFI-C · D-Limonene Cooling

Specimen SS-F-CLB-646 // COOL BURN · IFI-C

D-Limonene Cooling I-PR-B

Citrus sinensis

Orange

Tier ID-Limonene Cooling

Target Trait

1.15×

fold-change

D-limonene accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-646

Genomic Edits

4

GC Content

48.5%

Construct Length

2,733 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPM8

IFI Score

115

Pathway Edits

  • LS (limonene synthase) codon-optimized overexpression in Citrus sinensis flavedo
  • GPPS (geranyl pyrophosphate synthase) upregulation for GPP precursor flux
  • DXS (1-deoxy-D-xylulose 5-phosphate synthase) MEP pathway rate-limiting amplification
  • HDR (4-hydroxy-3-methylbut-2-enyl diphosphate reductase) MEP pathway co-upregulation

Key Interventions

D-limonene TRPM8-weak/TRPA1-dual overproduction via LS/GPPS co-upregulation in Citrus sinensisMEP pathway amplification via DXS/HDR for enhanced IPP/DMAPP precursor availabilityTRPM8/TRPA1 dual-modality cooling activation with characteristic citrus aroma co-production

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
115
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Citrus flavor enhancement, D-limonene ingredient sourcing, citrus-cooling beverage formulation, and functional food applications requiring natural TRPM8/TRPA1 dual-activation with citrus character.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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