IFI-C · Menthone Accumulation

Specimen SS-F-CLB-528 // COOL BURN · IFI-C

Menthone Accumulation I-IV-B

Mentha × piperita

Peppermint

Tier IMenthone Accumulation

Target Trait

1.49×

fold-change

menthone accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-528

Genomic Edits

4

GC Content

46.0%

Construct Length

2,727 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

149

Pathway Edits

  • PS (pulegone synthase) upregulation to increase pulegone intermediate flux toward menthone
  • L6H (limonene-6-hydroxylase) overexpression for enhanced hydroxylation toward menthol precursors
  • PIPR-RNAi (piperitone reductase RNAi) knockdown to prevent piperitone branch competition
  • GPPS (geranyl pyrophosphate synthase) monoterpene precursor upregulation cassette
  • MNPQ (menthone 9-reductase) loss-of-expression to prevent menthone-to-menthol conversion

Key Interventions

Menthone accumulation via PIPR knockdown and PS/L6H co-upregulation in Mentha × piperitaMNPQ suppression to preserve menthone pool rather than converting to downstream mentholTRPM8 precursor compound elevation with distinct minty-camphoraceous cooling character

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
149
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Menthone ingredient development, peppermint flavor diversification, rapid-onset cooling formulation, and applications requiring camphoraceous-minty cooling character distinct from classical menthol.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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