IFI-C · Isopulegol

Specimen SS-F-CLB-514 // COOL BURN · IFI-C

Isopulegol V-AG-B

Mentha × piperita

Peppermint

Tier VIsopulegol

Target Trait

5.29×

fold-change

isopulegol accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-514

Genomic Edits

10

GC Content

47.5%

Construct Length

5,093 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPM8

IFI Score

529

Pathway Edits

  • GLS (geraniol linalool synthase) overexpression cassette for isopulegol precursor flux
  • MFS1 (menthofuran synthase 1) loss-of-function knockout to eliminate competing pathway
  • GPPS (geranyl pyrophosphate synthase) upregulation for GPP substrate supply
  • TPS-ment (menthol-selective terpene synthase) variant edit for isopulegol branch specificity
  • MR (menthol reductase) suppression to preserve isopulegol accumulation vs. menthol conversion
  • Pulegone reductase PR variant swap for isopulegol-selective reduction step

Key Interventions

Isopulegol potent TRPM8 agonist overproduction via GLS/GPPS/TPS-ment engineeringMFS1 and MR suppression to redirect flux toward isopulegol rather than menthofuran/mentholElevated isopulegol accumulation for superior TRPM8 binding potency versus L-menthol

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
529
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Industrial high-potency TRPM8 agonist supply chain, clinical cooling compound research sourcing, ultra-concentration cooling ingredient development, and regulatory-grade isopulegol production programs.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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