IFI-C · Isopulegol

Specimen SS-F-CLB-504 // COOL BURN · IFI-C

Isopulegol III-IV-B

Mentha × piperita

Peppermint

Tier IIIIsopulegol

Target Trait

3.68×

fold-change

isopulegol accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-504

Genomic Edits

8

GC Content

48.9%

Construct Length

4,301 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

368

Pathway Edits

  • GLS (geraniol linalool synthase) overexpression cassette for isopulegol precursor flux
  • MFS1 (menthofuran synthase 1) loss-of-function knockout to eliminate competing pathway
  • GPPS (geranyl pyrophosphate synthase) upregulation for GPP substrate supply
  • TPS-ment (menthol-selective terpene synthase) variant edit for isopulegol branch specificity
  • MR (menthol reductase) suppression to preserve isopulegol accumulation vs. menthol conversion
  • Pulegone reductase PR variant swap for isopulegol-selective reduction step

Key Interventions

Isopulegol potent TRPM8 agonist overproduction via GLS/GPPS/TPS-ment engineeringMFS1 and MR suppression to redirect flux toward isopulegol rather than menthofuran/mentholElevated isopulegol accumulation for superior TRPM8 binding potency versus L-menthol

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
368
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium isopulegol ingredient supply, clinical TRPM8 research applications, reduced-dose cooling formulation for pharmaceutical and food uses, and next-generation high-potency cooling ingredient platforms.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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