IFI-C · Piperitone Cooling

Specimen SS-F-CLB-472 // COOL BURN · IFI-C

Piperitone Cooling IV-IV-B

Mentha spicata

Spearmint

Tier IVPiperitone Cooling

Target Trait

2.74×

fold-change

piperitone accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-472

Genomic Edits

6

GC Content

46.2%

Construct Length

3,628 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

274

Pathway Edits

  • TPS3 (terpene synthase 3, piperitone-selective) codon-optimized overexpression
  • GPPS (geranyl pyrophosphate synthase) upregulation for monoterpene precursor
  • CYP71D15 (limonene hydroxylase) stereoselective variant for piperitone branch flux
  • PIPR (piperitone reductase) loss-of-function RNAi to prevent piperitone consumption

Key Interventions

Piperitone monoterpenone overproduction via TPS3/GPPS co-upregulation in Mentha spicataTRPM8 agonist accumulation through CYP71D15 stereoselective hydroxylation optimizationPIPR suppression to prevent piperitone reduction and preserve TRPM8-active compound pool

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
274
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Industrial piperitone crop production, novel non-menthol cooling ingredient supply chains, clinical TRPM8 research ingredient programs, and next-generation earthy-mint flavor platform development.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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