IFI-C · Piperitone Cooling

Specimen SS-F-CLB-455 // COOL BURN · IFI-C

Piperitone Cooling II-IV-A

Mentha spicata

Spearmint

Tier IIPiperitone Cooling

Target Trait

1.52×

fold-change

piperitone accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-455

Genomic Edits

4

GC Content

45.4%

Construct Length

2,917 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

152

Pathway Edits

  • TPS3 (terpene synthase 3, piperitone-selective) codon-optimized overexpression
  • GPPS (geranyl pyrophosphate synthase) upregulation for monoterpene precursor
  • CYP71D15 (limonene hydroxylase) stereoselective variant for piperitone branch flux
  • PIPR (piperitone reductase) loss-of-function RNAi to prevent piperitone consumption

Key Interventions

Piperitone monoterpenone overproduction via TPS3/GPPS co-upregulation in Mentha spicataTRPM8 agonist accumulation through CYP71D15 stereoselective hydroxylation optimizationPIPR suppression to prevent piperitone reduction and preserve TRPM8-active compound pool

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
152
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Spearmint flavor diversification, piperitone ingredient development, earthy-mint cooling formulation, and functional food applications requiring non-menthol TRPM8 agonist compounds.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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