IFI-C · Pulegone Reduction

Specimen SS-F-CLB-415 // COOL BURN · IFI-C

Pulegone Reduction II-IV-A

Mentha × piperita

Peppermint

Tier IIPulegone Reduction

Target Trait

2.25×

fold-change

pulegone-to-menthol conversion fold-change

Technical Specifications

SKU

SS-F-CLB-415

Genomic Edits

5

GC Content

45.4%

Construct Length

3,217 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

225

Pathway Edits

  • PMR (pulegone reductase) overexpression cassette for pulegone-to-menthone conversion
  • PIPR (piperitone reductase) upregulation to redirect piperitone flux toward menthone
  • PS (pulegone synthase) loss-of-expression via trichome-specific RNAi knockdown
  • L6H (limonene-6-hydroxylase) precision upregulation for sustained pulegone substrate supply
  • MNPQ (menthone 9-reductase) co-upregulation for complete menthol pathway flux

Key Interventions

Pulegone-to-menthol pathway flux redirection via PMR/PIPR co-upregulationPS suppression to reduce toxic pulegone accumulation and redirect toward TRPM8 agonistComplete menthol biosynthesis cascade optimization for elevated cooling receptor activation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
225
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Safety-optimized peppermint ingredient supply, low-pulegone mint oil production, pharmaceutical-safe menthol ingredient development, and food-grade peppermint formulations with reduced hepatotoxic risk.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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