IFI-C · L-Menthol Biosynthesis

Specimen SS-F-CLB-232 // COOL BURN · IFI-C

L-Menthol Biosynthesis IV-IV-B

Mentha × piperita

Peppermint

Tier IVL-Menthol Biosynthesis

Target Trait

4.31×

fold-change

L-menthol accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-232

Genomic Edits

9

GC Content

46.2%

Construct Length

4,688 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

431

Pathway Edits

  • GPPS (geranyl pyrophosphate synthase) codon-optimized overexpression cassette
  • LS (limonene synthase) gain-of-expression via trichome-specific promoter swap
  • L6H (limonene-6-hydroxylase) precision upregulation for pulegone flux elevation
  • MNPQ (menthone 9-reductase) full-length coding sequence insertion
  • MNR (menthone reductase) stereoselectivity-optimized variant integration
  • PIPR (piperitone reductase) loss-of-function suppression via RNAi
  • TPS2 (terpene synthase 2) flux amplification cassette for monoterpene scaffold boost

Key Interventions

L-menthol pathway flux maximization via GPPS/LS/MNPQ co-upregulationStereoselective menthol accumulation through MNR variant engineeringTRPM8 receptor activation potency increase via elevated menthol tissue concentration

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
431
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Ultra-high-yield L-menthol crop engineering, industrial menthol ingredient supply chain development, clinical cooling ingredient research, and next-generation mint cultivar development programs.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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