IFI-C · L-Menthol Biosynthesis

Specimen SS-F-CLB-215 // COOL BURN · IFI-C

L-Menthol Biosynthesis II-IV-A

Mentha × piperita

Peppermint

Tier IIL-Menthol Biosynthesis

Target Trait

2.25×

fold-change

L-menthol accumulation fold-change

Technical Specifications

SKU

SS-F-CLB-215

Genomic Edits

5

GC Content

45.4%

Construct Length

3,217 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro T7/SP6 cell-free

Host Strain

Cell-free

Receptor Target

TRPM8

IFI Score

225

Pathway Edits

  • GPPS (geranyl pyrophosphate synthase) codon-optimized overexpression cassette
  • LS (limonene synthase) gain-of-expression via trichome-specific promoter swap
  • L6H (limonene-6-hydroxylase) precision upregulation for pulegone flux elevation
  • MNPQ (menthone 9-reductase) full-length coding sequence insertion
  • MNR (menthone reductase) stereoselectivity-optimized variant integration
  • PIPR (piperitone reductase) loss-of-function suppression via RNAi
  • TPS2 (terpene synthase 2) flux amplification cassette for monoterpene scaffold boost

Key Interventions

L-menthol pathway flux maximization via GPPS/LS/MNPQ co-upregulationStereoselective menthol accumulation through MNR variant engineeringTRPM8 receptor activation potency increase via elevated menthol tissue concentration

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
225
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Entry-level menthol ingredient supply, peppermint extract fortification, oral care product development, and confectionery cooling formulations requiring validated L-menthol elevation.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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