Specimen SS-F-BTR-999 // ZERO-BITTER · IFI-B
Broad-TAS2R · T5 Cell-Free-A
Brassica oleracea
Broccoli/Kale
Target Trait
85%
% reduction
Multi-axis TAS2R ligand reduction
Technical Specifications
SKU
SS-F-BTR-999
Genomic Edits
12
GC Content
45.3%
Construct Length
5,898 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
85
Pathway Edits
- MYB28 RNAi suppresses master transcriptional regulator of aliphatic glucosinolate biosynthesis globally
- ANR RNAi silencing reduces proanthocyanidin/condensed tannin production across all branch units
- TGG1 myrosinase enhancement improves glucosinolate hydrolysis fidelity, depleting the intact GLS ligand pool
- GSL-OH RNAi eliminates 2-hydroxy glucosinolate (progoitrin) accumulation from residual aliphatic GLS
- CYP79B2 RNAi reduces indole GLS (neoglucobrassicin) flux in parallel with aliphatic suppression
- Phenolic acid flux throttle limits chlorogenic acid co-accumulation that would sustain bitter phenolic load
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Brassica oleracea lines representing the most comprehensive commercially available TAS2R palatability improvement, suitable for new-to-category consumers, pediatric nutrition products, culinary innovation programs, and clinical trials where full Brassica bitter compound co-suppression is a study design requirement.
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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