Specimen SS-F-BTR-975 // ZERO-BITTER · IFI-B
Broad-TAS2R · T2 Cell-Free-A
Brassica oleracea
Broccoli/Kale
Target Trait
59%
% reduction
Multi-axis TAS2R ligand reduction
Technical Specifications
SKU
SS-F-BTR-975
Genomic Edits
8
GC Content
45.4%
Construct Length
4,277 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
59
Pathway Edits
- MYB28 RNAi suppresses master transcriptional regulator of aliphatic glucosinolate biosynthesis globally
- ANR RNAi silencing reduces proanthocyanidin/condensed tannin production across all branch units
- TGG1 myrosinase enhancement improves glucosinolate hydrolysis fidelity, depleting the intact GLS ligand pool
- GSL-OH RNAi eliminates 2-hydroxy glucosinolate (progoitrin) accumulation from residual aliphatic GLS
- CYP79B2 RNAi reduces indole GLS (neoglucobrassicin) flux in parallel with aliphatic suppression
- Phenolic acid flux throttle limits chlorogenic acid co-accumulation that would sustain bitter phenolic load
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier II Brassica oleracea lines representing the most comprehensive commercially available TAS2R palatability improvement, suitable for new-to-category consumers, pediatric nutrition products, culinary innovation programs, and clinical trials where full Brassica bitter compound co-suppression is a study design requirement.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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