Specimen SS-F-BTR-959 // ZERO-BITTER · IFI-B
Cucurbitacin · T5 Cell-Free-A
Solanum tuberosum
Potato
Target Trait
85%
% reduction
Cucurbitacin triterpenoid reduction
Technical Specifications
SKU
SS-F-BTR-959
Genomic Edits
9
GC Content
45.3%
Construct Length
4,678 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
85
Pathway Edits
- Bi bitter-gene RNAi eliminates the cucurbitacin B/E biosynthesis regulator controlling cucurbitacin pathway flux
- CYP81Q RNAi suppresses hydroxylation steps required for cucurbitacin ring activation and TAS2R binding
- GT-KO glycosyltransferase knockout blocks glycosylated cucurbitacin stabilization that extends tissue persistence
- Squalene epoxidase pathway throttle reduces total triterpenoid substrate pool available to the cucurbitacin branch
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Solanum tuberosum lines for programs targeting cucurbitacin elimination in potato breeding, Solanaceae-family functional food applications, and academic research into triterpenoid TAS2R activation where validated low-cucurbitacin germplasm is required as a study reference.
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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