Specimen SS-F-BTR-944 // ZERO-BITTER · IFI-B
Cucurbitacin · T3 Cell-Free-B
Solanum tuberosum
Potato
Target Trait
65%
% reduction
Cucurbitacin triterpenoid reduction
Technical Specifications
SKU
SS-F-BTR-944
Genomic Edits
7
GC Content
48.9%
Construct Length
4,001 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
65
Pathway Edits
- Bi bitter-gene RNAi eliminates the cucurbitacin B/E biosynthesis regulator controlling cucurbitacin pathway flux
- CYP81Q RNAi suppresses hydroxylation steps required for cucurbitacin ring activation and TAS2R binding
- GT-KO glycosyltransferase knockout blocks glycosylated cucurbitacin stabilization that extends tissue persistence
- Squalene epoxidase pathway throttle reduces total triterpenoid substrate pool available to the cucurbitacin branch
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III Solanum tuberosum lines for programs targeting cucurbitacin elimination in potato breeding, Solanaceae-family functional food applications, and academic research into triterpenoid TAS2R activation where validated low-cucurbitacin germplasm is required as a study reference.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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