Specimen SS-F-BTR-929 // ZERO-BITTER · IFI-B
Cucurbitacin · T2 Agrobacterium-A
Solanum tuberosum
Potato
Target Trait
55%
% reduction
Cucurbitacin triterpenoid reduction
Technical Specifications
SKU
SS-F-BTR-929
Genomic Edits
5
GC Content
45.2%
Construct Length
3,155 bp
Promoter System
35S CaMV / mas2
Expression System
T-DNA binary vector (pCAMBIA)
Host Strain
Agrobacterium tumefaciens EHA105
Receptor Target
TAS2R38 (suppression)
IFI Score
55
Pathway Edits
- Bi bitter-gene RNAi eliminates the cucurbitacin B/E biosynthesis regulator controlling cucurbitacin pathway flux
- CYP81Q RNAi suppresses hydroxylation steps required for cucurbitacin ring activation and TAS2R binding
- GT-KO glycosyltransferase knockout blocks glycosylated cucurbitacin stabilization that extends tissue persistence
- Squalene epoxidase pathway throttle reduces total triterpenoid substrate pool available to the cucurbitacin branch
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier II Solanum tuberosum lines for programs targeting cucurbitacin elimination in potato breeding, Solanaceae-family functional food applications, and academic research into triterpenoid TAS2R activation where validated low-cucurbitacin germplasm is required as a study reference.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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