Specimen SS-F-BTR-920 // ZERO-BITTER · IFI-B
Lactucopicrin · T5 Cell-Free-B
Brassica oleracea
Broccoli/Kale
Target Trait
78%
% reduction
Sesquiterpene bitter (lactucopicrin) reduction
Technical Specifications
SKU
SS-F-BTR-920
Genomic Edits
8
GC Content
47.4%
Construct Length
4,435 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
78
Pathway Edits
- GAS germacrene A synthase RNAi blocks sesquiterpene backbone formation upstream of lactucopicrin
- TLS (+)-trans-lactonase modification reduces lactucopicrin lactone ring stability and accumulation
- TLGS RNAi silences taraxacin lignan synthase branch producing lactucopicrin sesquiterpene precursors
- Mevalonate pathway throttle reduces total isoprenoid substrate pool available for sesquiterpene entry
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Brassica oleracea lines with reduced sesquiterpene lactone burden for applications in fresh-cut vegetables, dehydrated greens, and functional ingredient concentrates where lactucopicrin-associated bitterness detracts from product palatability and consumer acceptance.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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