IFI-B · Lactucopicrin Reduction Series

Specimen SS-F-BTR-917 // ZERO-BITTER · IFI-B

Lactucopicrin · T5 Protoplast-A

Brassica oleracea

Broccoli/Kale

Tier VLactucopicrin Reduction Series

Target Trait

78%

% reduction

Sesquiterpene bitter (lactucopicrin) reduction

Technical Specifications

SKU

SS-F-BTR-917

Genomic Edits

8

GC Content

44.4%

Construct Length

4,384 bp

Promoter System

35S CaMV / RbcS

Expression System

PEG-mediated protoplast transfection

Host Strain

Isolated leaf mesophyll protoplasts

Receptor Target

TAS2R38 (suppression)

IFI Score

78

Pathway Edits

  • GAS germacrene A synthase RNAi blocks sesquiterpene backbone formation upstream of lactucopicrin
  • TLS (+)-trans-lactonase modification reduces lactucopicrin lactone ring stability and accumulation
  • TLGS RNAi silences taraxacin lignan synthase branch producing lactucopicrin sesquiterpene precursors
  • Mevalonate pathway throttle reduces total isoprenoid substrate pool available for sesquiterpene entry

Key Interventions

GAS sesquiterpene synthase RNAiTLS lactone modificationTLGS precursor silencing

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
78
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V Brassica oleracea lines with reduced sesquiterpene lactone burden for applications in fresh-cut vegetables, dehydrated greens, and functional ingredient concentrates where lactucopicrin-associated bitterness detracts from product palatability and consumer acceptance.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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