Specimen SS-F-BTR-894 // ZERO-BITTER · IFI-B
Lactucopicrin · T2 Protoplast-B
Brassica oleracea
Broccoli/Kale
Target Trait
50%
% reduction
Sesquiterpene bitter (lactucopicrin) reduction
Technical Specifications
SKU
SS-F-BTR-894
Genomic Edits
4
GC Content
46.8%
Construct Length
2,780 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
50
Pathway Edits
- GAS germacrene A synthase RNAi blocks sesquiterpene backbone formation upstream of lactucopicrin
- TLS (+)-trans-lactonase modification reduces lactucopicrin lactone ring stability and accumulation
- TLGS RNAi silences taraxacin lignan synthase branch producing lactucopicrin sesquiterpene precursors
- Mevalonate pathway throttle reduces total isoprenoid substrate pool available for sesquiterpene entry
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier II Brassica oleracea lines with reduced sesquiterpene lactone burden for applications in fresh-cut vegetables, dehydrated greens, and functional ingredient concentrates where lactucopicrin-associated bitterness detracts from product palatability and consumer acceptance.
License
$749 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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