IFI-B · Lactucopicrin Reduction Series

Specimen SS-F-BTR-889 // ZERO-BITTER · IFI-B

Lactucopicrin · T2 Agrobacterium-A

Brassica oleracea

Broccoli/Kale

Tier IILactucopicrin Reduction Series

Target Trait

50%

% reduction

Sesquiterpene bitter (lactucopicrin) reduction

Technical Specifications

SKU

SS-F-BTR-889

Genomic Edits

4

GC Content

45.2%

Construct Length

2,895 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

50

Pathway Edits

  • GAS germacrene A synthase RNAi blocks sesquiterpene backbone formation upstream of lactucopicrin
  • TLS (+)-trans-lactonase modification reduces lactucopicrin lactone ring stability and accumulation
  • TLGS RNAi silences taraxacin lignan synthase branch producing lactucopicrin sesquiterpene precursors
  • Mevalonate pathway throttle reduces total isoprenoid substrate pool available for sesquiterpene entry

Key Interventions

GAS sesquiterpene synthase RNAiTLS lactone modificationTLGS precursor silencing

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
50
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier II Brassica oleracea lines with reduced sesquiterpene lactone burden for applications in fresh-cut vegetables, dehydrated greens, and functional ingredient concentrates where lactucopicrin-associated bitterness detracts from product palatability and consumer acceptance.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Zero-Bitter · IFI-BAll Modalities