Prenylflavonoid · T1 Agrobacterium-B

Humulus lupulus

Hops

Tier IPrenylflavonoid Reduction Series

Target Trait

36%

% reduction

Isoxanthohumol/8-PN reduction

Technical Specifications

SKU

SS-F-BTR-842

Genomic Edits

GC Content

45.8%

Construct Length

2,485 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

Pathway Edits

OMT1 O-methyltransferase RNAi reduces xanthohumol-to-isoxanthohumol Cope rearrangement substrate availability
PrenylT prenyltransferase RNAi limits prenylation of naringenin chalcone at the C-8 position
CHS2 chalcone synthase isoform modification reduces xanthohumol precursor pool from phloroglucyl-CoA

Key Interventions

OMT1 methyltransferase RNAiPrenylT C-8 prenylase silencingCHS2 chalcone reduction

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I Humulus lupulus lines for brewery applications targeting soft bitterness profiles, cosmetic and nutraceutical hop extracts requiring controlled 8-PN content, and non-alcoholic hop-flavored beverages where prenylflavonoid bitterness would detract from consumer palatability.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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