Specimen SS-F-BTR-822 // ZERO-BITTER · IFI-B
Coumarin Reduc · T3 Protoplast-B
Vitis vinifera
Grape
Target Trait
50%
% reduction
Coumarin/furocoumarin reduction
Technical Specifications
SKU
SS-F-BTR-822
Genomic Edits
5
GC Content
47.6%
Construct Length
3,167 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
50
Pathway Edits
- F6H1 feruloyl-CoA 6'-hydroxylase RNAi blocks scopoletin-to-coumarin lactone conversion
- CYP98A caffeic acid 3-O-methyltransferase modification reduces feruloyl substrate availability for the coumarin branch
- scopoletin-GT glucosyltransferase overexpression stabilizes scopoletin as non-bitter scopolin glucoside
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III Vitis vinifera lines with reduced coumarin and furocoumarin content for wine varieties, table grape applications, and grape extract formulations where bitter or medicinal coumarin flavor notes are undesirable quality defects.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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