IFI-B · Coumarin Reduction Series

Specimen SS-F-BTR-807 // ZERO-BITTER · IFI-B

Coumarin Reduc · T1 Cell-Free-A

Vitis vinifera

Grape

Tier ICoumarin Reduction Series

Target Trait

32%

% reduction

Coumarin/furocoumarin reduction

Technical Specifications

SKU

SS-F-BTR-807

Genomic Edits

3

GC Content

44.7%

Construct Length

2,490 bp

Promoter System

T7 / SP6 RNA polymerase

Expression System

Cell-free in vitro transcription/translation

Host Strain

In vitro wheat germ extract

Receptor Target

TAS2R38 (suppression)

IFI Score

32

Pathway Edits

  • F6H1 feruloyl-CoA 6'-hydroxylase RNAi blocks scopoletin-to-coumarin lactone conversion
  • CYP98A caffeic acid 3-O-methyltransferase modification reduces feruloyl substrate availability for the coumarin branch
  • scopoletin-GT glucosyltransferase overexpression stabilizes scopoletin as non-bitter scopolin glucoside

Key Interventions

F6H1 hydroxylase RNAiCYP98A feruloyl modificationscopoletin-GT enhancement

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
32
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier I Vitis vinifera lines with reduced coumarin and furocoumarin content for wine varieties, table grape applications, and grape extract formulations where bitter or medicinal coumarin flavor notes are undesirable quality defects.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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