Specimen SS-F-BTR-799 // ZERO-BITTER · IFI-B
Phlobaphene · T5 Cell-Free-A
Brassica oleracea
Broccoli/Kale
Target Trait
76%
% reduction
Phlobaphene (reddish-brown bitter) reduction
Technical Specifications
SKU
SS-F-BTR-799
Genomic Edits
7
GC Content
45.3%
Construct Length
3,998 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
76
Pathway Edits
- SG4 RNAi suppresses phlobaphene polymerization from flavan-4-ol units in epidermal cells
- LAR leucoanthocyanidin reductase modification reduces flavan-3,4-diol substrate availability for SG4
- ANR RNAi silencing limits anthocyanidin precursor flux that feeds the phlobaphene branch
- TT12 tonoplast transporter RNAi reduces phlobaphene sequestration and vacuolar stabilization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Brassica oleracea lines with reduced phlobaphene content for applications requiring clean flavor profiles in cooked Brassica products, dehydrated vegetable powders, and functional ingredient lines where off-color and bitter tannin co-presence is penalized.
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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