IFI-B · Phlobaphene Suppression Series

Specimen SS-F-BTR-789 // ZERO-BITTER · IFI-B

Phlobaphene · T4 Protoplast-A

Brassica oleracea

Broccoli/Kale

Tier IVPhlobaphene Suppression Series

Target Trait

67%

% reduction

Phlobaphene (reddish-brown bitter) reduction

Technical Specifications

SKU

SS-F-BTR-789

Genomic Edits

6

GC Content

43.4%

Construct Length

3,617 bp

Promoter System

35S CaMV / RbcS

Expression System

PEG-mediated protoplast transfection

Host Strain

Isolated leaf mesophyll protoplasts

Receptor Target

TAS2R38 (suppression)

IFI Score

67

Pathway Edits

  • SG4 RNAi suppresses phlobaphene polymerization from flavan-4-ol units in epidermal cells
  • LAR leucoanthocyanidin reductase modification reduces flavan-3,4-diol substrate availability for SG4
  • ANR RNAi silencing limits anthocyanidin precursor flux that feeds the phlobaphene branch
  • TT12 tonoplast transporter RNAi reduces phlobaphene sequestration and vacuolar stabilization

Key Interventions

SG4 polymerase RNAiLAR substrate reductionANR precursor silencing

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
67
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV Brassica oleracea lines with reduced phlobaphene content for applications requiring clean flavor profiles in cooked Brassica products, dehydrated vegetable powders, and functional ingredient lines where off-color and bitter tannin co-presence is penalized.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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