Specimen SS-F-BTR-784 // ZERO-BITTER · IFI-B
Phlobaphene · T3 Cell-Free-B
Brassica oleracea
Broccoli/Kale
Target Trait
57%
% reduction
Phlobaphene (reddish-brown bitter) reduction
Technical Specifications
SKU
SS-F-BTR-784
Genomic Edits
5
GC Content
48.9%
Construct Length
3,121 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
57
Pathway Edits
- SG4 RNAi suppresses phlobaphene polymerization from flavan-4-ol units in epidermal cells
- LAR leucoanthocyanidin reductase modification reduces flavan-3,4-diol substrate availability for SG4
- ANR RNAi silencing limits anthocyanidin precursor flux that feeds the phlobaphene branch
- TT12 tonoplast transporter RNAi reduces phlobaphene sequestration and vacuolar stabilization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III Brassica oleracea lines with reduced phlobaphene content for applications requiring clean flavor profiles in cooked Brassica products, dehydrated vegetable powders, and functional ingredient lines where off-color and bitter tannin co-presence is penalized.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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