IFI-B · Phlobaphene Suppression Series

Specimen SS-F-BTR-780 // ZERO-BITTER · IFI-B

Phlobaphene · T3 Biolistic-B

Brassica oleracea

Broccoli/Kale

Tier IIIPhlobaphene Suppression Series

Target Trait

57%

% reduction

Phlobaphene (reddish-brown bitter) reduction

Technical Specifications

SKU

SS-F-BTR-780

Genomic Edits

5

GC Content

46.5%

Construct Length

3,253 bp

Promoter System

UBI-1 / 35S CaMV

Expression System

Biolistic particle bombardment

Host Strain

Gold particle 0.6 µm

Receptor Target

TAS2R38 (suppression)

IFI Score

57

Pathway Edits

  • SG4 RNAi suppresses phlobaphene polymerization from flavan-4-ol units in epidermal cells
  • LAR leucoanthocyanidin reductase modification reduces flavan-3,4-diol substrate availability for SG4
  • ANR RNAi silencing limits anthocyanidin precursor flux that feeds the phlobaphene branch
  • TT12 tonoplast transporter RNAi reduces phlobaphene sequestration and vacuolar stabilization

Key Interventions

SG4 polymerase RNAiLAR substrate reductionANR precursor silencing

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
57
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III Brassica oleracea lines with reduced phlobaphene content for applications requiring clean flavor profiles in cooked Brassica products, dehydrated vegetable powders, and functional ingredient lines where off-color and bitter tannin co-presence is penalized.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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