IFI-B · Phlobaphene Suppression Series

Specimen SS-F-BTR-766 // ZERO-BITTER · IFI-B

Phlobaphene · T1 Protoplast-B

Brassica oleracea

Broccoli/Kale

Tier IPhlobaphene Suppression Series

Target Trait

38%

% reduction

Phlobaphene (reddish-brown bitter) reduction

Technical Specifications

SKU

SS-F-BTR-766

Genomic Edits

3

GC Content

48.5%

Construct Length

2,393 bp

Promoter System

35S CaMV / RbcS

Expression System

PEG-mediated protoplast transfection

Host Strain

Isolated leaf mesophyll protoplasts

Receptor Target

TAS2R38 (suppression)

IFI Score

38

Pathway Edits

  • SG4 RNAi suppresses phlobaphene polymerization from flavan-4-ol units in epidermal cells
  • LAR leucoanthocyanidin reductase modification reduces flavan-3,4-diol substrate availability for SG4
  • ANR RNAi silencing limits anthocyanidin precursor flux that feeds the phlobaphene branch
  • TT12 tonoplast transporter RNAi reduces phlobaphene sequestration and vacuolar stabilization

Key Interventions

SG4 polymerase RNAiLAR substrate reductionANR precursor silencing

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
38
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier I Brassica oleracea lines with reduced phlobaphene content for applications requiring clean flavor profiles in cooked Brassica products, dehydrated vegetable powders, and functional ingredient lines where off-color and bitter tannin co-presence is penalized.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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