Specimen SS-F-BTR-757 // ZERO-BITTER · IFI-B
ITC-Bitter Bal · T5 Protoplast-A
Raphanus sativus
Radish
Target Trait
72%
% reduction
Bitter GLS precursor reduction
Technical Specifications
SKU
SS-F-BTR-757
Genomic Edits
8
GC Content
44.4%
Construct Length
4,264 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
72
Pathway Edits
- GSL-OH RNAi silencing blocks 2-hydroxylation of 4-methylthiobutyl GLS in radish bitter precursor pathway
- AOP3 engineering redirects GLS hydrolysis products toward AITC-producing isothiocyanate channel
- TGG2 myrosinase isoform enhancement improves hydrolysis specificity toward ITC products
- ESP knockout eliminates nitrile/thiocyanate-forming specifier to bias entirely toward ITC production
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Raphanus sativus lines with optimized ITC-to-bitter ratio for fresh-market radish, daikon processing, and fermentation applications where hot/pungent ITC flavor is desired but TAS2R38-activating GLS bitterness should be minimized.
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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