Specimen SS-F-BTR-725 // ZERO-BITTER · IFI-B
ITC-Bitter Bal · T1 Protoplast-A
Raphanus sativus
Radish
Target Trait
35%
% reduction
Bitter GLS precursor reduction
Technical Specifications
SKU
SS-F-BTR-725
Genomic Edits
3
GC Content
43.9%
Construct Length
2,496 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
35
Pathway Edits
- GSL-OH RNAi silencing blocks 2-hydroxylation of 4-methylthiobutyl GLS in radish bitter precursor pathway
- AOP3 engineering redirects GLS hydrolysis products toward AITC-producing isothiocyanate channel
- TGG2 myrosinase isoform enhancement improves hydrolysis specificity toward ITC products
- ESP knockout eliminates nitrile/thiocyanate-forming specifier to bias entirely toward ITC production
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier I Raphanus sativus lines with optimized ITC-to-bitter ratio for fresh-market radish, daikon processing, and fermentation applications where hot/pungent ITC flavor is desired but TAS2R38-activating GLS bitterness should be minimized.
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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