Specimen SS-F-BTR-718 // ZERO-BITTER · IFI-B
Tannic Acid · T5 Protoplast-B
Vitis vinifera
Grape
Target Trait
65%
% reduction
High-MW tannin reduction
Technical Specifications
SKU
SS-F-BTR-718
Genomic Edits
7
GC Content
46.9%
Construct Length
4,021 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
65
Pathway Edits
- ANR anthocyanidin reductase RNAi reduces catechin/epicatechin extension units available for tannin polymerization
- TT12 tonoplast multidrug transporter RNAi impairs vacuolar sequestration and stabilization of condensed tannins
- LAR leucoanthocyanidin reductase RNAi reduces (+)-catechin monomer supply for proanthocyanidin polymerization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Vitis vinifera lines with reduced condensed tannin load for palatable table grape production, low-astringency wine varieties, and grape-derived polyphenol extracts where TAS2R activation is undesirable.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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