Specimen SS-F-BTR-710 // ZERO-BITTER · IFI-B
Tannic Acid · T4 Protoplast-B
Vitis vinifera
Grape
Target Trait
56%
% reduction
High-MW tannin reduction
Technical Specifications
SKU
SS-F-BTR-710
Genomic Edits
6
GC Content
49.3%
Construct Length
3,674 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
56
Pathway Edits
- ANR anthocyanidin reductase RNAi reduces catechin/epicatechin extension units available for tannin polymerization
- TT12 tonoplast multidrug transporter RNAi impairs vacuolar sequestration and stabilization of condensed tannins
- LAR leucoanthocyanidin reductase RNAi reduces (+)-catechin monomer supply for proanthocyanidin polymerization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV Vitis vinifera lines with reduced condensed tannin load for palatable table grape production, low-astringency wine varieties, and grape-derived polyphenol extracts where TAS2R activation is undesirable.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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