Specimen SS-F-BTR-704 // ZERO-BITTER · IFI-B
Tannic Acid · T3 Cell-Free-B
Vitis vinifera
Grape
Target Trait
48%
% reduction
High-MW tannin reduction
Technical Specifications
SKU
SS-F-BTR-704
Genomic Edits
5
GC Content
48.9%
Construct Length
3,161 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
48
Pathway Edits
- ANR anthocyanidin reductase RNAi reduces catechin/epicatechin extension units available for tannin polymerization
- TT12 tonoplast multidrug transporter RNAi impairs vacuolar sequestration and stabilization of condensed tannins
- LAR leucoanthocyanidin reductase RNAi reduces (+)-catechin monomer supply for proanthocyanidin polymerization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III Vitis vinifera lines with reduced condensed tannin load for palatable table grape production, low-astringency wine varieties, and grape-derived polyphenol extracts where TAS2R activation is undesirable.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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