Specimen SS-F-BTR-669 // ZERO-BITTER · IFI-B
Oleuropein · T4 Protoplast-A
Vitis vinifera
Grape
Target Trait
63%
% reduction
Oleuropein secoiridoid reduction
Technical Specifications
SKU
SS-F-BTR-669
Genomic Edits
7
GC Content
43.4%
Construct Length
3,957 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
63
Pathway Edits
- OleSyn oleuropein biosynthetic synthase RNAi reduces secoiridoid precursor availability in the iridoid pathway
- GES geraniol synthase RNAi limits geraniol supply at the entry step of the iridoid branch
- GT1 glucosyltransferase modification prevents oleuropein glucosylation stabilization in vacuoles
- betaGlu beta-glucosidase engineering accelerates oleuropein catabolism to non-bitter hydroxytyrosol products
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV Vitis vinifera lines with reduced secoiridoid bitter load for table grape breeding, wine fermentation where minimal oleuropein-derived bitterness is specified, and health-food applications requiring polyphenol palatability without compromising bioactive phenol content.
License
$3999 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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