Specimen SS-F-BTR-664 // ZERO-BITTER · IFI-B
Oleuropein · T3 Cell-Free-B
Vitis vinifera
Grape
Target Trait
54%
% reduction
Oleuropein secoiridoid reduction
Technical Specifications
SKU
SS-F-BTR-664
Genomic Edits
6
GC Content
48.9%
Construct Length
3,661 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
54
Pathway Edits
- OleSyn oleuropein biosynthetic synthase RNAi reduces secoiridoid precursor availability in the iridoid pathway
- GES geraniol synthase RNAi limits geraniol supply at the entry step of the iridoid branch
- GT1 glucosyltransferase modification prevents oleuropein glucosylation stabilization in vacuoles
- betaGlu beta-glucosidase engineering accelerates oleuropein catabolism to non-bitter hydroxytyrosol products
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III Vitis vinifera lines with reduced secoiridoid bitter load for table grape breeding, wine fermentation where minimal oleuropein-derived bitterness is specified, and health-food applications requiring polyphenol palatability without compromising bioactive phenol content.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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