IFI-B · Oleuropein Reduction Series

Specimen SS-F-BTR-661 // ZERO-BITTER · IFI-B

Oleuropein · T3 Protoplast-A

Vitis vinifera

Grape

Tier IIIOleuropein Reduction Series

Target Trait

54%

% reduction

Oleuropein secoiridoid reduction

Technical Specifications

SKU

SS-F-BTR-661

Genomic Edits

6

GC Content

45.1%

Construct Length

3,610 bp

Promoter System

35S CaMV / RbcS

Expression System

PEG-mediated protoplast transfection

Host Strain

Isolated leaf mesophyll protoplasts

Receptor Target

TAS2R38 (suppression)

IFI Score

54

Pathway Edits

  • OleSyn oleuropein biosynthetic synthase RNAi reduces secoiridoid precursor availability in the iridoid pathway
  • GES geraniol synthase RNAi limits geraniol supply at the entry step of the iridoid branch
  • GT1 glucosyltransferase modification prevents oleuropein glucosylation stabilization in vacuoles
  • betaGlu beta-glucosidase engineering accelerates oleuropein catabolism to non-bitter hydroxytyrosol products

Key Interventions

OleSyn synthase RNAiGES geraniol synthase blockbetaGlu enhancement

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
54
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III Vitis vinifera lines with reduced secoiridoid bitter load for table grape breeding, wine fermentation where minimal oleuropein-derived bitterness is specified, and health-food applications requiring polyphenol palatability without compromising bioactive phenol content.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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