Specimen SS-F-BTR-646 // ZERO-BITTER · IFI-B
Oleuropein · T1 Protoplast-B
Vitis vinifera
Grape
Target Trait
35%
% reduction
Oleuropein secoiridoid reduction
Technical Specifications
SKU
SS-F-BTR-646
Genomic Edits
3
GC Content
48.5%
Construct Length
2,353 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
35
Pathway Edits
- OleSyn oleuropein biosynthetic synthase RNAi reduces secoiridoid precursor availability in the iridoid pathway
- GES geraniol synthase RNAi limits geraniol supply at the entry step of the iridoid branch
- GT1 glucosyltransferase modification prevents oleuropein glucosylation stabilization in vacuoles
- betaGlu beta-glucosidase engineering accelerates oleuropein catabolism to non-bitter hydroxytyrosol products
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier I Vitis vinifera lines with reduced secoiridoid bitter load for table grape breeding, wine fermentation where minimal oleuropein-derived bitterness is specified, and health-food applications requiring polyphenol palatability without compromising bioactive phenol content.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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