Specimen SS-F-BTR-640 // ZERO-BITTER · IFI-B
Glycoalkaloid · T5 Cell-Free-B
Solanum tuberosum
Potato
Target Trait
85%
% reduction
Solanine/chaconine reduction
Technical Specifications
SKU
SS-F-BTR-640
Genomic Edits
9
GC Content
47.4%
Construct Length
4,655 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
85
Pathway Edits
- SGT1 solanidine glucosyltransferase RNAi blocks galactosylation step in α-solanine terminal glycosylation
- SGT3 solanidine glucosyltransferase RNAi eliminates rhamnose addition step in α-chaconine pathway
- GAME12 RNAi suppresses solanidine-to-tomatidine conversion branch that generates chaconine precursors
- GAME4 modification reduces overall steroidal alkaloid pathway throughput at the cycloartenol-to-solanidine axis
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Solanum tuberosum lines with reduced glycoalkaloid content for fresh-market potato production, potato-derived food ingredients, and breeding programs targeting improved consumer palatability and regulatory compliance with glycoalkaloid thresholds.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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