Specimen SS-F-BTR-629 // ZERO-BITTER · IFI-B
Glycoalkaloid · T4 Protoplast-A
Solanum tuberosum
Potato
Target Trait
75%
% reduction
Solanine/chaconine reduction
Technical Specifications
SKU
SS-F-BTR-629
Genomic Edits
8
GC Content
43.4%
Construct Length
4,257 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
75
Pathway Edits
- SGT1 solanidine glucosyltransferase RNAi blocks galactosylation step in α-solanine terminal glycosylation
- SGT3 solanidine glucosyltransferase RNAi eliminates rhamnose addition step in α-chaconine pathway
- GAME12 RNAi suppresses solanidine-to-tomatidine conversion branch that generates chaconine precursors
- GAME4 modification reduces overall steroidal alkaloid pathway throughput at the cycloartenol-to-solanidine axis
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV Solanum tuberosum lines with reduced glycoalkaloid content for fresh-market potato production, potato-derived food ingredients, and breeding programs targeting improved consumer palatability and regulatory compliance with glycoalkaloid thresholds.
License
$3999 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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