IFI-B · Glycoalkaloid Reduction Series

Specimen SS-F-BTR-617 // ZERO-BITTER · IFI-B

Glycoalkaloid · T3 Agrobacterium-A

Solanum tuberosum

Potato

Tier IIIGlycoalkaloid Reduction Series

Target Trait

65%

% reduction

Solanine/chaconine reduction

Technical Specifications

SKU

SS-F-BTR-617

Genomic Edits

7

GC Content

43.7%

Construct Length

4,042 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

65

Pathway Edits

  • SGT1 solanidine glucosyltransferase RNAi blocks galactosylation step in α-solanine terminal glycosylation
  • SGT3 solanidine glucosyltransferase RNAi eliminates rhamnose addition step in α-chaconine pathway
  • GAME12 RNAi suppresses solanidine-to-tomatidine conversion branch that generates chaconine precursors
  • GAME4 modification reduces overall steroidal alkaloid pathway throughput at the cycloartenol-to-solanidine axis

Key Interventions

SGT1 galactosyltransferase RNAiSGT3 rhamnosyltransferase silencingGAME12 suppression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
65
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III Solanum tuberosum lines with reduced glycoalkaloid content for fresh-market potato production, potato-derived food ingredients, and breeding programs targeting improved consumer palatability and regulatory compliance with glycoalkaloid thresholds.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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