Specimen SS-F-BTR-600 // ZERO-BITTER · IFI-B
Limonin Reduc · T5 Cell-Free-B
Citrus sinensis
Orange
Target Trait
70%
% reduction
Limonin reduction
Technical Specifications
SKU
SS-F-BTR-600
Genomic Edits
7
GC Content
47.4%
Construct Length
4,015 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
70
Pathway Edits
- LAS limonoid A-ring synthase RNAi reduces obacunone-to-limonin triterpenoid conversion
- UDPG-GT UDP-glucuronosyltransferase overexpression promotes limonin glucosylation to non-bitter 17β-glucoside
- COSY limonoid biosynthetic pathway throttle reduces total triterpenoid flux entering the limonin branch
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Citrus sinensis lines for juice processing operations requiring reduced delayed-onset bitterness, premium juice products where limonin development during storage is a quality defect, and low-bitter citrus extract formulations.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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