IFI-B · Limonin Reduction Series

Specimen SS-F-BTR-586 // ZERO-BITTER · IFI-B

Limonin Reduc · T4 Agrobacterium-B

Citrus sinensis

Orange

Tier IVLimonin Reduction Series

Target Trait

61%

% reduction

Limonin reduction

Technical Specifications

SKU

SS-F-BTR-586

Genomic Edits

6

GC Content

46.7%

Construct Length

3,566 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

61

Pathway Edits

  • LAS limonoid A-ring synthase RNAi reduces obacunone-to-limonin triterpenoid conversion
  • UDPG-GT UDP-glucuronosyltransferase overexpression promotes limonin glucosylation to non-bitter 17β-glucoside
  • COSY limonoid biosynthetic pathway throttle reduces total triterpenoid flux entering the limonin branch

Key Interventions

LAS triterpenoid synthase RNAiUDPG-GT glucosyltransferase overexpressionCOSY pathway throttle

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
61
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV Citrus sinensis lines for juice processing operations requiring reduced delayed-onset bitterness, premium juice products where limonin development during storage is a quality defect, and low-bitter citrus extract formulations.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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