Specimen SS-F-BTR-576 // ZERO-BITTER · IFI-B
Limonin Reduc · T2 Cell-Free-B
Citrus sinensis
Orange
Target Trait
44%
% reduction
Limonin reduction
Technical Specifications
SKU
SS-F-BTR-576
Genomic Edits
4
GC Content
47.3%
Construct Length
2,774 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
44
Pathway Edits
- LAS limonoid A-ring synthase RNAi reduces obacunone-to-limonin triterpenoid conversion
- UDPG-GT UDP-glucuronosyltransferase overexpression promotes limonin glucosylation to non-bitter 17β-glucoside
- COSY limonoid biosynthetic pathway throttle reduces total triterpenoid flux entering the limonin branch
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier II Citrus sinensis lines for juice processing operations requiring reduced delayed-onset bitterness, premium juice products where limonin development during storage is a quality defect, and low-bitter citrus extract formulations.
License
$749 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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