IFI-B · Limonin Reduction Series

Specimen SS-F-BTR-569 // ZERO-BITTER · IFI-B

Limonin Reduc · T2 Agrobacterium-A

Citrus sinensis

Orange

Tier IILimonin Reduction Series

Target Trait

44%

% reduction

Limonin reduction

Technical Specifications

SKU

SS-F-BTR-569

Genomic Edits

4

GC Content

45.2%

Construct Length

2,855 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

44

Pathway Edits

  • LAS limonoid A-ring synthase RNAi reduces obacunone-to-limonin triterpenoid conversion
  • UDPG-GT UDP-glucuronosyltransferase overexpression promotes limonin glucosylation to non-bitter 17β-glucoside
  • COSY limonoid biosynthetic pathway throttle reduces total triterpenoid flux entering the limonin branch

Key Interventions

LAS triterpenoid synthase RNAiUDPG-GT glucosyltransferase overexpressionCOSY pathway throttle

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
44
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier II Citrus sinensis lines for juice processing operations requiring reduced delayed-onset bitterness, premium juice products where limonin development during storage is a quality defect, and low-bitter citrus extract formulations.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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