Specimen SS-F-BTR-558 // ZERO-BITTER · IFI-B
Naringin Reduc · T5 Protoplast-B
Citrus sinensis
Orange
Target Trait
80%
% reduction
Naringin reduction
Technical Specifications
SKU
SS-F-BTR-558
Genomic Edits
8
GC Content
46.9%
Construct Length
4,281 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
80
Pathway Edits
- RhaT rhamnosyltransferase RNAi prevents final rhamnosylation of naringenin-7-glucoside to bitter naringin
- GT1 glucosyltransferase alternative isoform redirects naringenin to non-bitter glycoside conjugates
- CHS chalcone synthase RNAi reduces total naringenin precursor pool available for glycosylation
- CHI chalcone isomerase modification channels chalcone flux away from naringenin accumulation
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Citrus sinensis lines with validated naringin reduction for fresh juice processing, citrus-flavored beverages, and functional food applications where grapefruit-like bitterness would otherwise activate TAS2R38 to objectionable levels.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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